Rose-Marie Amini’s research projects on malignant lymphoma

Molecular alterations in marginal zone lymphoma

One of our projects focuses on marginal zone lymphoma, a low malignant B-cell lymphoma that often is difficult to diagnose. We have access to tissues from about 100 patients and we aim to identify specific molecular changes on marginal zone lymphoma to more separate them from other types of B-cell lymphoma. We also try to find molecular characteristics that are associated with variations in clinical presentation and to the risk that the disease is transformed into a more aggressive form.
 

Microenvironment and mutations in DLBCL

   Cells in Diffuse large B-cell
   lymphoma (DLBCL).

We also study a clinically very aggressive type of B-cell lymphoma called Diffuse Large B-Cell Lymphoma (DLBCL). This is a heterogeneous group of lymphoma where the genetic characterisation of the tumour is important for determining which therapy is the best for the individual patient.

In the project we immunohistochemically stain patient tumour material to identify inflammatory cells. The goal is to map the role of inflammatory components in the tumour microenvironment. We also sequence DNA from the tissues to study mutations in the tumours that are involved in lymphoma development and prognosis.
 

T-cell lymphoma with varying clinical progression

Another aggressive lymphoma type is T-prolymphocyte leukemia, which is a distinctive and quite rare kind of T-cell lymphoma where patients seldom survive for more than a year. However, there are variants of the disease with a milder clinical development and it is important to determine the reasons for this difference.

In this study we analyse lymphoma cells from approximately 20 patients where material has been collected both at diagnosis and at follow-ups. This is used to study genetic mutations and clonal evolution using targeted sequencing (Haloplex technology)